Review

atf4 (Santa Cruz Biotechnology)

Santa Cruz Biotechnology is a verified supplier

About

News

Press Release

Team

Advisors

Partners

Contact

Bioz Stars

Bioz vStars

Buy from Supplier

Structured Review

Santa Cruz Biotechnology atf4
Atf4, supplied by Santa Cruz Biotechnology, used in various techniques. Bioz Stars score: 96/100, based on 956 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/atf4/ATF-4+Antibody/pm42854627-61-42-43
Average 96 stars, based on 956 article reviews

atf4 - by Bioz Stars, 2026-11

96/100 stars

Images

Cell-Signaling:

Article Title: Shear stress-induced Ca 2+ influx triggers endoplasmic reticulum stress and cardiomyocyte apoptosis: implications for mitral regulation
Article Snippet: After protein separation, the gels were transferred onto Immobilon-E PVDF Membranes (Millipore, IEVH85R) and blocked with F1 1-min Blocking Buffer Plus (Lion Biotechnologies Inc., F1BLOK500P). .. The samples were analyzed using antibodies against Grp78 (BD, 610978), ATF6 (Santa Cruz, sc166659), p-PERK (Cell Signaling, 3179), PERK (Cell Signaling, 3192), p-IRE1α (Abcam, ab48187), IRE1α (Abcam, ab37073), p-eIF2α (Cell Signaling, 9721), eIF2α (Cell Signaling, 9722), ATF4 (Santa Cruz, sc390063), CHOP (Cell Signaling, 2895), Bax (Genetex, GTX109683), Bcl-2 (BD, 610538), Bak (Cell Signaling, 12105), caspase 9 (Santa Cruz, sc56076), caspase 12 (Cell Signaling, 35965), caspase 3 (Abcam, ab32042), caspase 8 (Proteintech, 13423-1-AP), Bid (Novus, 10988-1-AP), and transient receptor potential canonical 1 (TRPC1) (Santa Cruz, sc133076). .. GAPDH (Abcam, ab8245) was used as a loading control.

Article Title: IRP1 affects the long-term survival and function of iTregs by regulating ferroptosis and apoptosis.
Article Snippet: Equal amounts of protein (20 μg/lane) were separated by SDS-PAGE and transferred onto PVDF membranes (Millipore, USA; #IPVH00010). .. Membranes were blocked with 5% non-fat milk in TBST for 1 h at room temperature and incubated overnight at 4 ◦C with primary antibodies against IRP1 (Abcam, UK; ab126595; 1:1000), GPX4 (Cell Signaling Technology, USA; #52455S; 1:2000), CHAC1 (Proteintech, China; #28265–1-AP; 1:800), ATF4 (Santa Cruz Biotechnology, USA; sc390063; 1:1000), ATF3 (Cell Signaling Technology, USA; #33593S; 1:500), CHOP (Cell Signaling Technology, USA; #5554S; 1:1000), and β-actin (Sigma-Aldrich, USA; #A2228; 1:5000). .. After washing with TBST, membranes were incubated with HRPconjugated secondary antibodies (Jackson ImmunoResearch, USA; 1:5000) for 1 h at room temperature.

Incubation:

Article Title: Targeting FOSL2 via knockdown: a druggable strategy for protecting against hepatic ischemia/reperfusion injury through ATF4 suppression.
Article Snippet: Macrophage-mediated inflammatory response is a critical pathogenic component in hepatic ischemia/reperfusion injury (HIRI) development.. Although FOS-like antigen 2 (FOSL2) plays a pivotal role in regulating macrophage polarization, its role and potential mechanisms in HIRI remain unclear.. The findings demonstrated that FOSL2 was increased in both the liver tissues and macrophages of the liver tissues in IR rats.

Staining:

Article Title: Magnetic field-induced ER stress reprograms the tumor microenvironment to improve triple-negative breast cancer survival
Article Snippet: Cells were then fixed and permeabilized using fixation/permeability kit per manufacturer’s protocol (BD Biosciences Cytofix/CytopermTM Kit #554715). .. Intracellular staining was performed using the following primary antibodies: GRP78-Alexa Fluor 647 (ThermoFisher #CL647-66574), CHOP-PE (Santa Cruz Biotechnology #sc-56107 PE), ATF4 (Santa Cruz Biotechnology #sc-390063 AF594), and xBP1s-Alexa Fluor 488 (ProteinTech #CL488-24868). .. Flow cytometry acquisition was performed on an Invitrogen Attune NXT flow cytometer, and data were analyzed using De Novo software (FCS Express).

other:

Article Title: Shear stress-induced Ca 2+ influx triggers endoplasmic reticulum stress and cardiomyocyte apoptosis: implications for mitral regulation.
Article Snippet: We quantified the protein content of the samples and then separated the proteins based on molecular weight via sodium dodecyl sulfate‒polyacrylamide gel electrophoresis (SDS‒PAGE) on 7.5, 12.5, and 15% gels using an electrophoresis tank (Bio-Rad).

Immunohistochemistry:

Article Title: Investigation of molecular effects of beta caryophyllene in cadmium-induced nephrotoxicity model in rats: in silico and in vivo studies.
Article Snippet: Background: Cadmium is one of the toxic heavy metals that accumulate in the kidneys, and it can cause kidney damage by triggering oxidative stress, ER stress, inflammation, and apoptosis.. β-caryophyllene (BCP) is a volatile ingredient found in various spice essential oils, known for its anti-inflammatory and strong oxygen-free radical scavenging properties.. In this study, we aimed to test the effects of BCP on kidney damage caused by Cd

Western Blot:

Article Title: Differential cell survival outcomes in response to diverse amino acid stress
Article Snippet: .. The following antibodies were used in immunoblotting experiments: phospho-4EBP1 (T37/46, 1:1,000, 2855; Cell Signaling), Atf4 (1:1,000, sc-390063; Santa Cruz), phospho-Gcn2 (T899, ab75836, 1:1,000; Abcam), Grb2 (1:1,000, 610112; BD Biosciences), puromycin (1:10,000, MABE343; Millipore), phospho-S6K (T389, 1:1,000, 9234; Cell Signaling), Tsc2 (1:1,000, 4308; Cell Signaling), vinculin (1:2,000, 66305-1-Ig; Proteintech). ..

Image Search Results

(A) Glioma cell survival at 24 h after SS treatment. (B) Glioma cell survival after pretreatment with DFO, FAC, Fer‐1, and Lip‐1, respectively, for 1 h before SS treatment. (C–F) Intracellular ROS, MDA, GSH/GSSG, and ferrous ion levels at 6, 12, and 24 h after SS treatment, with and without DFO, Fer‐1, Lip‐1, and FAC pretreatment for 1 h before SS treatment, respectively. (G) Glioma cell survival after SS treatment, with and without pretreatment with NAC and GSH for 1 h before SS treatment, respectively. (H) Western blots of ferroptosis‐related protein expression after SS treatment. Cell survival was assessed by the CCK‐8 assay. (I) Western blots of ATF3 expression in the cytoplasm and nuclei of SS‐treated cells. (J) Viability of cells treated with siATF3 followed by SS. (K–N) Ferrous ion, MDA, GSH/GSSG, and ROS levels in cells treated with siATF3 followed by SS. (O) Confocal microscopic images of ATF3 immunofluorescence in U251 cells treated with SS. (P, Q) Confocal microscopy images and fluorescence intensity measurements of mitochondrial superoxide in SS‐ and siATF3‐treated U251 and T98 cells. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Journal: CNS Neuroscience & Therapeutics

Article Title: ATF3 Promotes Solasonine‐Triggered Ferroptosis in Glioma Cells by Suppressing Glycolysis

doi: 10.1002/cns.71226

Figure Lengend Snippet: (A) Glioma cell survival at 24 h after SS treatment. (B) Glioma cell survival after pretreatment with DFO, FAC, Fer‐1, and Lip‐1, respectively, for 1 h before SS treatment. (C–F) Intracellular ROS, MDA, GSH/GSSG, and ferrous ion levels at 6, 12, and 24 h after SS treatment, with and without DFO, Fer‐1, Lip‐1, and FAC pretreatment for 1 h before SS treatment, respectively. (G) Glioma cell survival after SS treatment, with and without pretreatment with NAC and GSH for 1 h before SS treatment, respectively. (H) Western blots of ferroptosis‐related protein expression after SS treatment. Cell survival was assessed by the CCK‐8 assay. (I) Western blots of ATF3 expression in the cytoplasm and nuclei of SS‐treated cells. (J) Viability of cells treated with siATF3 followed by SS. (K–N) Ferrous ion, MDA, GSH/GSSG, and ROS levels in cells treated with siATF3 followed by SS. (O) Confocal microscopic images of ATF3 immunofluorescence in U251 cells treated with SS. (P, Q) Confocal microscopy images and fluorescence intensity measurements of mitochondrial superoxide in SS‐ and siATF3‐treated U251 and T98 cells. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Article Snippet: Transferrin receptor (TfR), transferrin, ferroportin (FPN), ferritin, GPX4, xCT, H2A, ß‐actin, ATG5, NCOA4, ferritin heavy chain (FTH), microtubule‐associated protein 1 light chain 3ß (LC3B), p62, pyruvate kinase M2 (PKM2), phosphofructokinase platelet type (PFKP), catalase, glucose‐regulated protein 78 (GRP78), protein kinase RNA–like endoplasmic reticulum kinase (PERK), ATF4, ATF3, and hexokinase 2 (HK2) antibodies were purchased from Abclonal (China).

Techniques: Western Blot, Expressing, CCK-8 Assay, Confocal, Immunofluorescence, Microscopy, Fluorescence, Control

(A) Western blots of ferritinophagy‐related protein expression in glioma cells after SS treatment. (B–E) Cell viability and ROS, MDA, and ferrous ion levels after pretreatment with 3MA and Baf‐A1 followed by SS treatment. (F) Western blots of ferroptosis‐ and ferritinophagy‐related protein expression in glioma cells after 3MA and Baf‐A1 pretreatment and SS treatment. (G) Viability of glioma cells treated with siATG5 followed by SS. (H–J) ROS, MDA, and ferrous ion levels in glioma cells treated with siATG5 followed by SS. (K, L) Western blots of ferritinophagy‐ and ferroptosis‐related protein expression in glioma cells treated with siATG5 and siATF3, respectively, followed by SS. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Journal: CNS Neuroscience & Therapeutics

Article Title: ATF3 Promotes Solasonine‐Triggered Ferroptosis in Glioma Cells by Suppressing Glycolysis

doi: 10.1002/cns.71226

Figure Lengend Snippet: (A) Western blots of ferritinophagy‐related protein expression in glioma cells after SS treatment. (B–E) Cell viability and ROS, MDA, and ferrous ion levels after pretreatment with 3MA and Baf‐A1 followed by SS treatment. (F) Western blots of ferroptosis‐ and ferritinophagy‐related protein expression in glioma cells after 3MA and Baf‐A1 pretreatment and SS treatment. (G) Viability of glioma cells treated with siATG5 followed by SS. (H–J) ROS, MDA, and ferrous ion levels in glioma cells treated with siATG5 followed by SS. (K, L) Western blots of ferritinophagy‐ and ferroptosis‐related protein expression in glioma cells treated with siATG5 and siATF3, respectively, followed by SS. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Techniques: Western Blot, Expressing, Control

(A) Western blots of antioxidant‐related protein expression after SS treatment. (B, C) The intracellular hydrogen peroxide level and antioxidant‐related protein expression after pretreatment with SP and ATP, respectively, followed by SS treatment. (D) Ferrous ion levels after hydrogen peroxide treatment. (E, F) Western blots of ferritinophagy‐related protein expression in hydrogen peroxide– and NAC‐treated cells. (G–I) ATP, PA, and hydrogen peroxide levels in glioma cells treated with siATF3 and SS. (J) Western blots of glycolysis‐related protein expression in glioma cells treated with siATF3 and SS. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Journal: CNS Neuroscience & Therapeutics

Article Title: ATF3 Promotes Solasonine‐Triggered Ferroptosis in Glioma Cells by Suppressing Glycolysis

doi: 10.1002/cns.71226

Figure Lengend Snippet: (A) Western blots of antioxidant‐related protein expression after SS treatment. (B, C) The intracellular hydrogen peroxide level and antioxidant‐related protein expression after pretreatment with SP and ATP, respectively, followed by SS treatment. (D) Ferrous ion levels after hydrogen peroxide treatment. (E, F) Western blots of ferritinophagy‐related protein expression in hydrogen peroxide– and NAC‐treated cells. (G–I) ATP, PA, and hydrogen peroxide levels in glioma cells treated with siATF3 and SS. (J) Western blots of glycolysis‐related protein expression in glioma cells treated with siATF3 and SS. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Techniques: Western Blot, Expressing, Control

(A–C) Western blots of ER stress–related protein expression after SS treatment with and without SP and ATP pretreatment, and after hydrogen peroxide treatment. (D–F) ROS, MDA, and ferrous ion levels in cells treated with SS after pretreatment with 4‐PBA. (G, H) Western blots of ferroptosis‐ and ER stress–related protein expression in cells treated with 4‐PBA and SS. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Journal: CNS Neuroscience & Therapeutics

Article Title: ATF3 Promotes Solasonine‐Triggered Ferroptosis in Glioma Cells by Suppressing Glycolysis

doi: 10.1002/cns.71226

Figure Lengend Snippet: (A–C) Western blots of ER stress–related protein expression after SS treatment with and without SP and ATP pretreatment, and after hydrogen peroxide treatment. (D–F) ROS, MDA, and ferrous ion levels in cells treated with SS after pretreatment with 4‐PBA. (G, H) Western blots of ferroptosis‐ and ER stress–related protein expression in cells treated with 4‐PBA and SS. * p < 0.01 versus control, # p < 0.01 versus treatment group.

Techniques: Western Blot, Expressing, Control

(A) Photograph of nude mouse tumors. (B) Tumor volume over time. (C–F) Hydrogen peroxide, MDA, ferrous ion, and GSH/GSSG levels in tumor tissues from nude mice treated with high and low doses of SS. (G) Western blots of glycolysis‐, ferritinophagy‐, and ferroptosis‐related protein expression in tumor tissues. (H) H&E staining of intracranial orthotopic tumor following SS treatment. (I) Expression of intratumoral NCOA4, PKM2, and GPX4 in each treatment group (bar = 50 μm). * p < 0.01 versus control.

Journal: CNS Neuroscience & Therapeutics

Article Title: ATF3 Promotes Solasonine‐Triggered Ferroptosis in Glioma Cells by Suppressing Glycolysis

doi: 10.1002/cns.71226

Figure Lengend Snippet: (A) Photograph of nude mouse tumors. (B) Tumor volume over time. (C–F) Hydrogen peroxide, MDA, ferrous ion, and GSH/GSSG levels in tumor tissues from nude mice treated with high and low doses of SS. (G) Western blots of glycolysis‐, ferritinophagy‐, and ferroptosis‐related protein expression in tumor tissues. (H) H&E staining of intracranial orthotopic tumor following SS treatment. (I) Expression of intratumoral NCOA4, PKM2, and GPX4 in each treatment group (bar = 50 μm). * p < 0.01 versus control.

Techniques: Western Blot, Expressing, Staining, Control

Schematic illustration of the fabrication and application of the WT-Exo–loaded coaxial nanofibrous membrane for rescuing p75NTR deficiency–associated osteogenic impairment and promoting bone regeneration.

Journal: Materials Today Bio

Article Title: EMSCs-derived exosomes rescue p75NTR −/− -mediated osteogenesis deficiency via PI3K/Akt/ERK1/2 signaling pathways

doi: 10.1016/j.mtbio.2026.103755

Figure Lengend Snippet: Schematic illustration of the fabrication and application of the WT-Exo–loaded coaxial nanofibrous membrane for rescuing p75NTR deficiency–associated osteogenic impairment and promoting bone regeneration.

Article Snippet: Osteogenesis-related antibodies, including anti-Col-I (A5786), anti-OPN (A1499), anti-activating transcription factor 4 (ATF4, A18687) and anti-ALP (A0514), were obtained from Abclonal Technology Co., Ltd. (Wuhan, China).

Techniques: Membrane

Gene expression profiles between (SF/PCL 2:5 /PVA) WT-Exo and SF/PCL 2:5 /PVA groups. (A) Volcano plot displaying differentially expressed genes between the (SF/PCL 2:5 /PVA) WT-Exo and SF/PCL 2:5 /PVA groups. Red dots represent upregulated genes, and blue dots represent downregulated genes. (B) Heatmap of selected differentially expressed genes. (C) KEGG pathway enrichment analysis. (D) Gene Ontology (GO) analysis showing enriched biological processes, cellular components and molecular functions. (E) Venn diagram showing the overlap of differentially expressed genes annotated to the PI3K/Akt signaling pathway, ERK1/2 signaling cascade, and osteoblast differentiation–related gene sets. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Journal: Materials Today Bio

Article Title: EMSCs-derived exosomes rescue p75NTR −/− -mediated osteogenesis deficiency via PI3K/Akt/ERK1/2 signaling pathways

doi: 10.1016/j.mtbio.2026.103755

Figure Lengend Snippet: Gene expression profiles between (SF/PCL 2:5 /PVA) WT-Exo and SF/PCL 2:5 /PVA groups. (A) Volcano plot displaying differentially expressed genes between the (SF/PCL 2:5 /PVA) WT-Exo and SF/PCL 2:5 /PVA groups. Red dots represent upregulated genes, and blue dots represent downregulated genes. (B) Heatmap of selected differentially expressed genes. (C) KEGG pathway enrichment analysis. (D) Gene Ontology (GO) analysis showing enriched biological processes, cellular components and molecular functions. (E) Venn diagram showing the overlap of differentially expressed genes annotated to the PI3K/Akt signaling pathway, ERK1/2 signaling cascade, and osteoblast differentiation–related gene sets. (For interpretation of the references to colour in this figure legend, the reader is referred to the Web version of this article.)

Techniques: Gene Expression, Analysis, Significance Assay

Journal: Materials Today Bio

Article Title: EMSCs-derived exosomes rescue p75NTR −/− -mediated osteogenesis deficiency via PI3K/Akt/ERK1/2 signaling pathways

doi: 10.1016/j.mtbio.2026.103755

Figure Lengend Snippet:

Techniques:

OGM and ERA induce ferroptosis reporter genes in ATRT cell lines. Relative expression of known OGM‐mediated ferroptosis reporter genes (A) ATF4, (B) HMOX1, (C) SLC7A11, and (D) TFRC in CHLA‐02‐ATRT and CHLA‐06‐ATRT lines treated with OGM or ERA. (E) Relative expression of a second housekeeping gene, ACTB, confirming that GAPDH expression is unaffected by OGM or ERA. (A–E) n = 3 biological repeats with n = 3 technical repeats. Graphs represent mean ± SD with significance determined by two‐tailed, equal variance, t ‐tests. Comparisons are between DMSO and treatments * p < 0.0125, ** p < 0.0025, and *** p < 0.00025.

Journal: Cancer Reports

Article Title: GPR68 Inhibition Induces Ferroptosis in Atypical Teratoid Rhabdoid Tumors

doi: 10.1002/cnr2.70719

Figure Lengend Snippet: OGM and ERA induce ferroptosis reporter genes in ATRT cell lines. Relative expression of known OGM‐mediated ferroptosis reporter genes (A) ATF4, (B) HMOX1, (C) SLC7A11, and (D) TFRC in CHLA‐02‐ATRT and CHLA‐06‐ATRT lines treated with OGM or ERA. (E) Relative expression of a second housekeeping gene, ACTB, confirming that GAPDH expression is unaffected by OGM or ERA. (A–E) n = 3 biological repeats with n = 3 technical repeats. Graphs represent mean ± SD with significance determined by two‐tailed, equal variance, t ‐tests. Comparisons are between DMSO and treatments * p < 0.0125, ** p < 0.0025, and *** p < 0.00025.

Article Snippet: Cells were transfected on 12‐well plates as described above with the ATF4 luciferase reporter plasmid from Addgene (plasmid #21850) [ ].

Techniques: Expressing, Two Tailed Test

Genetic validation of ferroptosis in CHLA‐06‐ATRT. (A) Treatment with OGM or ERA increases Bodipy 581/591 C11 oxidation, indicating increased lipid peroxidation. (B) Representative images from (A). (C) Expression of GFP demonstrates the transfectability of CHLA‐06‐ATRT cells. (D) Transfection with siRNA against GPR68 decreases cell viability (left) and the relative expression of GPR68 (right). (E) Overexpression of human GPR68 increases cell growth. (F) An ATF4 reporter plasmid indicates that treatment with OGM increases ATF4 expression. (G) Overexpression of human ATF4 is sufficient to decrease cell survival. (H) Transfection with siRNA against ATF4 rescues cell viability (left) and decreases the relative expression of ATF4 (right). (A) n ≥ 4 biological repeats with n ≥ 355 aggregated cells and 10 μM OGM or 15 μM ERA. (C) n = 4 biological repeats. (D) and (H) For cell survival, n = 8 biological repeats with n = 4 technical repeats, and for qRT‐PCR n = 3 biological repeats with n = 3 technical repeats. (H) 2 μM OGM. (E) and (G) n = 8 biological repeats with n = 4 technical repeats. (F) n = 6 biological repeats with n = 4 technical repeats and 2 μM OGM. (A) Mean ± SEM with significance determined by two‐tailed, equal variance, t ‐tests with *** p < 0.0005. (B, C) ×20 magnification. (D–H) Mean ± SD with significance determined by two‐tailed, equal variance, t‐tests. (D) Comparisons are between control and treatments *** p < 0.0005. (E–G) ** p < 0.01 and *** p < 0.001. (H) Five comparisons are between control and treatments, and ** p < 0.002 and *** p < 0.0002.

Journal: Cancer Reports

Article Title: GPR68 Inhibition Induces Ferroptosis in Atypical Teratoid Rhabdoid Tumors

doi: 10.1002/cnr2.70719

Figure Lengend Snippet: Genetic validation of ferroptosis in CHLA‐06‐ATRT. (A) Treatment with OGM or ERA increases Bodipy 581/591 C11 oxidation, indicating increased lipid peroxidation. (B) Representative images from (A). (C) Expression of GFP demonstrates the transfectability of CHLA‐06‐ATRT cells. (D) Transfection with siRNA against GPR68 decreases cell viability (left) and the relative expression of GPR68 (right). (E) Overexpression of human GPR68 increases cell growth. (F) An ATF4 reporter plasmid indicates that treatment with OGM increases ATF4 expression. (G) Overexpression of human ATF4 is sufficient to decrease cell survival. (H) Transfection with siRNA against ATF4 rescues cell viability (left) and decreases the relative expression of ATF4 (right). (A) n ≥ 4 biological repeats with n ≥ 355 aggregated cells and 10 μM OGM or 15 μM ERA. (C) n = 4 biological repeats. (D) and (H) For cell survival, n = 8 biological repeats with n = 4 technical repeats, and for qRT‐PCR n = 3 biological repeats with n = 3 technical repeats. (H) 2 μM OGM. (E) and (G) n = 8 biological repeats with n = 4 technical repeats. (F) n = 6 biological repeats with n = 4 technical repeats and 2 μM OGM. (A) Mean ± SEM with significance determined by two‐tailed, equal variance, t ‐tests with *** p < 0.0005. (B, C) ×20 magnification. (D–H) Mean ± SD with significance determined by two‐tailed, equal variance, t‐tests. (D) Comparisons are between control and treatments *** p < 0.0005. (E–G) ** p < 0.01 and *** p < 0.001. (H) Five comparisons are between control and treatments, and ** p < 0.002 and *** p < 0.0002.

Article Snippet: Cells were transfected on 12‐well plates as described above with the ATF4 luciferase reporter plasmid from Addgene (plasmid #21850) [ ].

Techniques: Biomarker Discovery, Expressing, Transfection, Over Expression, Plasmid Preparation, Quantitative RT-PCR, Two Tailed Test, Control

Review

atf4 (Santa Cruz Biotechnology)

Structured Review

Images

Related Articles

Cell-Signaling:

Incubation:

Staining:

other:

Immunohistochemistry:

Western Blot:

Similar Products

Image Search Results